Mikel Arteta will miss the Premier League match facing Manchester City on 1st January subsequently testing positive for Covid-19 for a second time.
Assistants Albert Stuivenberg and Steve Round will deal at Emirates Stadium.
Calum Chambers, Cedric Soares, Takehiro Tomiyasu and Ainsley Maitland-Niles missed the 5-0 victory over Norwich on 26th December after testing positive for Coronavirus Disease.
Arteta tested positive in March 2020 as well.
“Mikel is isolating in line with Government guidelines and we wish him well,” according to Arsenal statement.
Steven Gerrard, Aston Villa manager and Crystal Palace boss Patrick Vieira missed games over the Christmas period after positive tests.
Arsenal, who have triumphed their past four league matches, are fourth in the table, 12 points behind leaders City.
Pierre-Emerick Aubameyang, who has been missed for the past five games in all competitions following being stripped of the captaincy, will also overlook the City match.
He is in Dubai with the Gabon squad getting ready for the Africa Cup of Nations.